Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus

نویسندگان

  • Carla Sousa-Santos
  • Paulo J. Fonseca
  • Maria Clara P. Amorim
چکیده

The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.

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عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2015